Heat Resistance of ProteolyticClostridium botulinurnType F in Phosphate Buffer and Crabmeat

摘要

ABSTRACTThe heat resistance of proteolytic C.botulinumtype F strains Langeland, PC and 4YRC was studied by the thermal death time tube method in phosphate buffer; 4Y,RC was also studied in crabmeat. Correction of exposure time for thermal lag and lethality during lag was made by the graphic method. Decimal reduction times in minutes (D) were calculated by Schmidt's probability method, using the Karber LD50. For these 3 strains, D curves in phosphate buffer traversed one log cycle in 18.1–25.3°F (z); extrapolated they gave 0.14–0.23 for D250. The z of the 4YRC crabmeat D curve was 23, extrapolating to 0.17–0.18 for D250. Proteolytic type F, therefore, resembles other proteolyticC. botulinur