In Vitro Regeneration, Flowering and Seed Formation from Leaf Explants of Scoparia dulcis L.

摘要

This study describes an efficient protocol for the induction of in vitro flowering and fruiting of Scoparia dulcis L. (family -Scrophulariaceae), a multipurpose folk medicinal plant. Murashige and Skoog medium supplemented with kinetin (KIN; 13.93 uM)and indole-3-acetic acid (IAA; 1.14 uM) is optimal for the formation of multiple shoots (20.00 ± 1.67), which induced floral buds (13 ± 0.43). The regenerated plantlets developed flowers and fruits within 40-45 and 50-55 days, respectively. In vitro floral development was asynchronous. The morphology of the in vitro developed floral organs was almost similar to that of the parent plant. The flowers produced in vitro developed ripe fruits with viable seeds. Our finding has important implications in understanding the influence of physiological factors and the molecular basis of floral organ formation in this valuable medicinal plant.