Different sensitivity of various brain structures to thioacetamide-induced lipid peroxidation

摘要

Thioacetamide (TAA) exerts hepatotoxic, neurotoxic and carcinogenic effects. The aim of our study was to investigate the effects of TAA on lipid peroxidation and catalase activity in various rat brain regions. Male Wistar rats were divided into following groups: 1. control, saline-treated; 2. thioacetamide-treated groups, TAA 300 (300 mg/kg), TAA 600 (600 mg/kg) and TAA 900 (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA 300), twice (TAA 600) and three times (TAA 900) in consecutive days. Brain samples were collected 24 h after the last dose of TAA and malondialdehyde (MDA) level and catalase activity were determined in cortex, brainstem and hippocampus. MDA level was significantly increased while catalase activity was significantly lower in all brain regions in TAA 900 group in comparison with control group. In TAA 600 MDA level was increased in the brainstem and cortex when compared to control (p0.01). The same dose of TAA 600 mg/kg induced a significant decline in catalase activity in the brainstem and cortex and an increase in its activity in the hippocampus when compared to control (p0.01). In TAA 300 an increase in MDA level was evident only in the brainstem. Catalase activity was significantly higher in the cortex and hippocampus in TAA 300 group in comparison with control (p0.01). Based on these results, it may be concluded that various rat brain regions have different sensitivity to TAA-induced lipid peroxidation with hippocampus being less sensitive than cerebral cortex and brainstem.