In vitro biosynthesis and quantification of plumbagin in cell suspension culture of Plumbago zeylanica

摘要

Plumbagin is the major alkaloid responsible for medicinal activities of Plumbago zeylanica. Experiments were conducted to quantify secondary metabolite (plumbagin) production in calli / cell clumps/embryoid acquired from cell suspension culture of P.zeylanica. Higher plumbagin amount was detected in one-month-old calli / cell clumps/embryoid fraction (0.428mg/100gm), liquid culture medium fraction free of calli / cell clumps/embryoid (0.357 mgL~(-1)) acquired from cell suspension culture as well asin two-months-old rhizogenic calli (1.257 mg per gm) on culture medium MS3D.5IB ((MS+3.0 mgL~(-1) 2,4-D + 0.5 mgL~(-1) IBA). Linearly increased plumbagin concentration in both fractions of cell suspension culture was recorded with increased concentrationof 2,4-D. MS2D.5IB (MS+2.0 mgL~(-1) 2,4-D + 0.5 mgL~(-1) IBA) was found the best culture medium in increasing growth and rhizogenic calli response in liquid medium.