A new glucose biosensor was developed based on hollow carbon spheres decorated with platinum nanoparticles (Pt/HCSs)-modified glassy carbon electrode immobilized with glucose oxidase (GOx) with the help of Nafion. The Pt nanoparticles were well dispersed on the HCSs with an average size of 2.29 nm. The detection of glucose was achieved via electrochemical detection of the enzymatically liberated H _2O _2 at <0.5 V versus Ag/AgCl at physiologic pH of 7.4. The Pt/HCSs-modified electrode exhibited excellent electrocatalytic activities toward both the oxidation and reduction of H _2O _2. The glucose biosensor showed good electrocatalytic performance in terms of high sensitivity (4.1 lA μM ~(-1)), low detection limit (1.8 μM), fast response time3 s, and wide linear range (0.04-8.62 μM). The apparent Michaelis- Menten constant (Km) and the maximum current density (imax) values for the biosensor were 10.94 μM and 887 μA cm ~(-2) respectively. Furthermore, this biosensor showed an acceptable reproducibility and high stability. The interfering signals from ascorbic acid and uric acid at concentration levels normally found in human blood were not much compared with the response to glucose. Blood serum samples were also tested with this biosensor and a good recovery was achieved for the two spiked serum samples.
展开▼
