Scale-up of analytical chromatography to the simulated moving bed separation of the enantiomers of the flavour norterpenoids #alpha#-ionone and #alpha#-damascone

摘要

The scale-up of the HPLC separation of the enantiomers of the flavour norterpenoid ketones #alpha#-ionone and #alpha#-damascone from analytical to preparative scale was achieved using the chromatographic simulated moving bed (SMB) technique. Commercial HPLC columns, with heptakis-(2,3,6-per-O-methyl)-#beta#-cyclodextrin bound to silica gel as chiral stationary phase, were employed for the separation. High-purity enantiomers of both chiral compounds have been obtained with a rather satisfactory productivity.