Use of the SALMOTYPE R meat juice ELISA for the detection of salmonella antibodies in serum of slaughter pigs

摘要

The German Federal Ministry of Health and the Federal Ministry of Food, Agriculture and Forestry have presented "guidelines for a programme to reduce the introduction of salmonella into meat production by slaughter pigs". These guidelines intend todetermine the prevalence of salmonella in farms raising slaughter pigs. For this, random samples are to be examined with a meat juice ELISA to detect salmonella antibodies. In this study the use of two such ELISA systems was tested. In order to compare the commercially available SALMOTYPE meat juice ELISA with a meat juice ELISA developed by the German Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), test sera were examined in parallel via both systems. In these examinations the SALMOTYPE meat juice ELISA showed a higher sensitivity. Further experiments were carried out to analyse the practical applicability of the SALMOTYPE meat juice ELISA. For this, 119 serum samples of slaughter pigs were examined. The SALMOTYPEmeat juice ELISA was shown to be fast, easy to perform and reliable.