Improvement in inheritance of somatic embryogen-esis and plantlet regeneration in tropical maize lines from friable callus

摘要

Recalcitrance to regeneration and transformation of tropical maize has slowed down the potential research in improvement of variety, quality, and capability of tropical maize to withstand abiotic and biotic stresses. The Plant Transformation Laboratory (PTL) at Kenyatta University in Kenya has developed a regeneration system for tropical maize inbred lines important to Kenyan breeders by using 1 mm to 2 mm immature zygotic embryos as ex-plants. This system has proved to be highly genotype-dependent.In this study, inbred, single-cross hybrid and backcross generations were developed from crossing maize inbred CML216 with commercially important inbred maize line TL08.