Standard operating procedure for metabonomics studies of blood serum and plasma samples using a 1H-NMR micro-flow probe

摘要

A standard operating procedure (SOP) is presented for high-throughput metabonomics studies using a 600 MHz NMRspectrometer equipped with a micro-flow probe that is connected to an auto-sampler. The procedure is designed to minimizerandom and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the dataacquired by a given NMR spectroscopist to ensure that (i) all researchers involved in the NMR data acquisition of a metabonomicsresearch program perform equally and (ii) operator-associated variation of NMR data collection is statistically not relevant forthe interpretation of results obtained from multivariate data analyses.