Synthesis and characterization of hydrophilic lipopolymers for the support of lipid bilayers

摘要

This paper describes a flexible synthetic route to build-up lipopolymers. This is achieved on the basis of copolymers from different acrylamides and the acrylate of N-hydroxysuccinimide. Through reactions on the polymers (polymer-analogous reactions) with DMPE (2-dimyristyl-L-#alpha#-phosphatidyloxy)ethylamine) and 2-aminoethyl methyl disulfide the terpolymer composition can be varied easily. The polymers obtained by this method can be used to prepare self-assembled polymer films on gold-coated substrates. A characterization of the self-assembled film and of the polymeric monolayer at the water surface demonstrates the importance of the hydrophilic comonomer. Polymers including the more hydrophilic N,N-dimethylacrylamide allow to vary the hydrophilicity of the self-assembled films to a large extent (contact angles varying from 35deg to 72deg depending on the amount of phospholipid). In addition, these films swell up to 25% in an atmosphere of 98% humidity. For obtaining hydrophilic swellable polymers to build-up tethered supported lipid bilayers they are therefore preferable.