Analysis of very long chain polyunsaturated fatty acids using high-performance liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

摘要

The presence and identity of very long chain polyunsaturated fatty acids from three freshwater crustacecan species, Bathynella natans, B. baicalensis and Baicalobathynella magna from Lake Baikal and caves of central Europe were determined by means ofliquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS with APCI). LC-MS with APCI enabled the identification of more than 50 very long chain polyunsaturated fatty acids. These acids were described in the crustaceans for the first time. predominantly 26:5n6, 28:7n6, 30:7n3 and 40:7n6. A hypothesis for the biosynthesis of these acids is proposed.