2-chloroacetaldehyde induces epsilon dA DNA adducts in DNA of Raji cells as demonstrated by an improved HPLC-fluorimetry method

摘要

Etheno adducts in DNA are promutagenic lesions and are formed from vinyl chloride, urethane, and lipid peroxidation-derived products such as 4-hydroxy-nonenal, Such adducts were induced in a B-lymphoid cell line (Raji) by a metabolite of vinyl chloride, 2-chloro-acetaldehyde. By modification of a method of Bedell et al,, etheno dA was determined in 20 mug DNA by HPLC-fluorimetry, Our method has a detection limit of 5 fmol which is one two-hundredth that of the original method. By use of this method initial evidence was found that cigarette smoke can also induce etheno dA adducts in Raji cell DNA. [References: 27]