Superoxide dismutase biosensors working in non-aqueous solvent

摘要

Enzyatmic electrodes based on superoxide dismutae enzyme were developed. Using the superoxide dismutase enzyme sensor assembled according to the classical model, poor results were obtained. Results were improved by adopting a new way of assembling the biosensor using a cellulose triacetate layer in which the SOD enzyme is entrapped and sandwiched between two gas-permeable membranes, or using a kappa-carrageenan gel layer entrapping the enzyme, sandwiched between an external gas permeable membrane and an internal cellulose acetates membrane, coupled in each case to the oxyen amperometric tranducer. Results obtained by applying the newly developed bisoensor to assaying hydrophobic compounds showing radical scavenging properties, operating in dmethylsulfoxide, were also satisfactory.