Rapid differentiation of Ophiostoma ulmi and Ophiostoma novo-ulmi isolates by matrix-assisted-laser-desorption/ionization time-of-flight/time-of-flight mass spectrometry

摘要

The intact yeast phase spores of Ophiostoma ulmi and O. novo-ulmi isolates were analysed by matrix-assisted-laser-desorption/ionization time-of-flight/time-of-flight mass spectrometry. The results clearly showed that isolates of O. ulmi and O. novo-ulmi have different mass peak profiles. The specific mass in m/z may vary slightly. One of the peaks from m/z 7623 to 7630 dominated (in high intensity) mass spectra for all isolates of O. novo-ulmi tested in this study. This specific peak is believed to be CU (cerato-ulmi) protein. However, no specific m/z peak can be found to distinguish between the isolates of ssp. novo-ulmi and ssp. americana. On the contrary, all isolates of O. ulmi tested possessed one of a dominate peak at m/z 7337 to 7342, but none around m/z 7626 as shown in O. novo-ulmi isolates.