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Genotyping of fimbrial adhesins in Escherichia coli strains isolated from Slovak piglets suffering from diarrhea

机译:从患有腹泻的斯洛伐克小猪中分离出的大肠杆菌菌株中纤维黏附素的基因分型

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摘要

One-hundred sixty Escherichia coli isolates obtained from piglets with diarrhea from different parts of Slovakia were examined for the presence of genes coding for F4, F5, F6 and F41 fimbrial adhesins, and hemolytic activity. According to polymerase chain reaction tests 74 (46 %) E. coli isolates were positive for primers that detected genes coding for fimbrial adhesins. Of these 74 isolates, 64 were positive for genes encoding for F4(+), four for F5(+), five for F6(+), and one for both F4(+) and F5(+) adhesins.
机译:检查从斯洛伐克不同地区腹泻的仔猪中分离出的一百六十株大肠杆菌,检测其编码F4,F5,F6和F41纤维粘附素的基因的存在以及溶血活性。根据聚合酶链反应测试,有74(46%)大肠杆菌分离株的引物呈阳性,该引物检测到编码纤维黏附素的基因。在这74个分离株中,有64个对编码F4(+)的基因呈阳性,四个对F5(+),五个对F6(+),一个对F4(+)和F5(+)粘附素。

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