Discordance at D3S1358 locus involving SGM Plus? and the European new generation multiplex kits

摘要

During the course of routine database sample analysis in the Israel Police DNA database, an off-ladder D3S1358 allele, calculated to be 22.1, extending into the adjacent vWA locus was observed using Applied Biosystems SGM Plus? kit. To verify the size of this D3S1358 long allele and to ensure it was not part of a trialle pattern in the neighboring locus, the sample was amplified using three of the European new generation STR multiplex kits: NGMTM (Applied Biosystem), Powerplex? ESX and ESI (Promega). The results of these amplifications determined the variant to be a 22 allele. Subsequent sequencing confirmed this designation and revealed a nucleotide polymorphism. Ten additional SGM Plus? profiled samples with D3S1358 alleles larger than 19, were re-analyzed using NGMTM and Powerplex? ESX which also showed discordance in the calculated results between original SGM Plus? designations and those obtained with the European new generation multiplexes.