Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples

摘要

Short tandem repeat (STR) DNA typing is a global standard for human identification. Current practice involves highly trained forensic analysts, operating in a laboratory setting, using multiple instruments to process samples and analyze the data. Here, we report the developmental validation of a fully integrated and automated DNA profiling system, the RapidHIT (R) System, capable of producing up to five high quality STR profiles with full controls in approximately 90 min using PowerPlex (R) 16 HS RapidHIT chemistry. The system integrates all sample handling steps: starting from lysis of cells on buccal swabs or other buccal sample types through DNA extraction, normalization, amplification, capillary array electrophoresis, detection, and integrated software analysis.