Differentiation of polycystic ovary syndrome-derived human embryonic stem cells into adipocytes and their glucose consumption

摘要

Background: Polycystic ovary syndrome (PCOS) is related to genetic factors. Adipose tissue and insulin resistance (IR) may play an important role in the pathogenesis and progression of PCOS. We investigate glucose consumption and insulin response abilities in the adipocytes differentiated from PCOS-derived human embryonic stem cells (hESCs) in vitro in order to provide a new idea for exploring PCOS pathogenesis. Methods: hESC lines were established with the discarded embryos of non-PCOS or PCOS-women, and then were differentiated into adipocytes. The glucose consumption ability and insulin response ability for these adipocytes were detected. Results: There was no significant difference in glucose consumption ability between non-PCOS and PCOS-derived adipocytes at the absence of insulin. Insulin could significantly increase glucose consumption abilities of both non-PCOS and PCOS-derived adipocytes, but there was no significant difference in the increased glucose consumption ability between the two types of adipocytes. Conclusion: The glucose consumption ability and insulin response ability in the PCOS-derived adipocytes are similar to that in non-PCOS-derived adipocytes.