Detection of banned antibacterial growth promoter in animal feed by enzyme-linked immunosorbent assay: Method validation according to the Commission Decision 2002/657/EC criteria

摘要

An enzyme-linked immunosorbent assay (ELISA) test has been applied for the qualitative screening analysis of virginiamycin and bacitracin in feedingstuffs at level of 1 mg/kg. The ELISA validation study was performed according to the Commission Decision 2002/657/EC criteria established for qualitative screening methods in food. In this regard, the following parameters were determined: detection capability (CC beta), specificity and ruggedness. The resulted CO values were <1 ppm for both molecules and no interferences from matrix effects were observed.