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首页> 外文期刊>Food Biotechnology >Development and Application of SCAR Marker for the Detection of Papaya Seed Adulteration in Traded Black Pepper Powder.
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Development and Application of SCAR Marker for the Detection of Papaya Seed Adulteration in Traded Black Pepper Powder.

机译:SCAR标记在交易黑胡椒粉中木瓜种子掺假检测中的开发和应用。

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摘要

The present study reports the development of a specific, sensitive, and reproducible Sequence Characterized Amplified Region (SCAR) marker to detect papaya seed powder adulteration in traded black pepper powder. A putative RAPD marker (449 bp) specific to papaya seed was identified, cloned, and sequenced to design the SCAR primers. This specific SCAR marker could detect the presence of papaya seed in all the analyzed simulated standards and in one of five branded market samples of black pepper powder tested. The analytical strategy being very simple could be used for large scale screening of powdered black pepper market samples intended for export and domestic uses.
机译:本研究报告了开发一种特异性,灵敏和可重复的序列特征扩增区域(SCAR)标记,以检测黑胡椒粉中木瓜籽粉的掺假情况。鉴定,克隆和测序特定于木瓜种子的推定的RAPD标记(449 bp),以设计SCAR引物。这种特定的SCAR标记可以在所有分析的模拟标准品中和在所测试的黑胡椒粉的五个品牌市场样品之一中检测到木瓜种子的存在。非常简单的分析策略可用于大规模筛选打算用于出口和国内用途的黑胡椒粉市场样品。

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