Effects of a partially digested whey protein concentrate on Salmonella enterica serotype Typhimurium adhesion to Caco-2 cells. Prevention of Salmonella adhesion to Caco-2 cells using whey.

摘要

The effect of a partially lipase-digested whey protein concentrate (WPD) containing free fatty acids on the adherence of Salmonella enterica serovar Typhimurium to Caco-2 cells was investigated. A short concurrent exposure of the Caco-2 monolayers and WPD caused dose-dependent inhibition of bacterial adhesion. Pre-treatment of Salmonella Typhimurium with WPD resulted in inhibition of adhesion equivalent to that with concurrent administration, whereas treatment with WPD did not significantly inhibit adhesion. WPD was not bactericidal towards Salmonella Typhimurium at up to 50mgml-1 for up to 1h, but was bacteriostatic over 12h. High concentrations of WPD (>20mgml-1) were cytotoxic to newly-confluent cell monolayers, depending on the duration of exposure. Lactoferrin (LF), caseinoglycomacropeptide (CGMP), and gamma globulins all significantly inhibited Salmonella Typhimurium adhesion