EVALUATION OF CHANGES IN METABOLIC HORMONES AND METABOLIC INDICES DURING PHYSIOLOGICAL AND PATHOLOGICAL COURSE OF PUERPERIUM IN LACTATING COWS

摘要

The metabolic hormones and metabolites are important mediators of reproduction and nutrition status of the cows. A total of 20 Slovak Holstein spotted breed multiparous cows were used for the study. Animals were allocated in two groups; first dairy cows with physiological course of puerperium (G_1) (n=11) and second group dairy cows with pathological course of puerperium (G_2) (n=9). Milk production in cows was 8568 +-428 kg.year~(-1) Blood samples for analysis of IGF-I, insulin, non-esterified fattyacid (NEFA), aspartat-aminotransferase (AST), alanin-aminotransferase (ALT),glucose and total cholestrerol (TCH) were collected twice weekly from 21 days antepartum. to S6 days postpartum. Significantly lower concentrations of IGF I in G_2 were found only during first and second weeks in postpartum period period (89.675 +- 11.5112 ng.ml~(-1) vs. 105-58 +-10.76 ng.ml~(-1) and 77.433 +- 7.226 ng.ml~(-1) vs. 91.5516 +- 10.0875 ng.ml~(-1)) (P < 0.05) Within five weeks in postpartal period were recorded significantly higher concentrations of insulin in G_1, (average 3.676 +- 0.08 ng.mll~(-1) vs. 3.12 +- 0.18 ng.ml~(-1)) (P < 0.01). Were found higher concentrations of NEFA in G_2 during eightweeks postpartum opposite within eight weeks postpartum (average 0.51+- 0034 mmol.l~(-1) versus 0.34 +- 0.029 ramol.l~(-1)) (P < 0.01). Were recorded significantly higher concentrations of AST during four weeks postpartum in G_2 (average 1.86 +-0.0315 mukat.l~(-1) vs. 1.67 +- 0.024 (ikat.l~(-1)) (P < 001). During late pregnancy and transition period have recorded significantly changes of metabolites concentrations between cows with different course of puerperium.