INTRASPECIES VARIABILITY OF INFRAPOPULATIONS OF Fascioloides magna BASED ON SELECTED VARIABLE SECTIONS COX1 AND NAD1 OF GENES OF MITOCHONDRIAL DNA

摘要

The study investigated genetic variability of Fascioloides magna within three infrapopulations originating from Canada, Slovakia and Croatia, based on sequences of variable sections of two genes of mitochondrial DNA, subunit 1 cytochrome oxidase (cox1) and nicotinamide dehydrogenase (nad1), by means of sequencing and the SSCP (Single Strand Conformational Polymorphism) method. Comparison of genetic structure of parasite infrapopulations from distant territories allowed us to assess for the first timethe extent of intraspecies variability of F. magna. Results of the research form a basis for more extensive phylogeographic study of the parasite and explanation of historic processes of its spreading.