Occurrence and seasonal variation of aflatoxin in dairy cow feed with estimation of aflatoxin M-1 in milk from Iran

摘要

The aims of the present study were to investigate the occurrence of aflatoxin B-1 (AFB(1)), aflatoxin B-2 (AFB(2)), aflatoxin G(1) (AFG(1)) and aflatoxin G(2) (AFG(2)) in dairy cow feedstuff samples collected from four west provinces in Iran and estimate the corresponding concentrations of aflatoxin M-1 (AFM(1)) in milk during winter and summer in 2014. A total of 160 feed samples consisting of corn silage (n=40), alfalfa hay (n=40), straw (n=40) and barley (n=40) were analyzed using high-performance liquid chromatography with a fluorescence detector method. AFB(1), AFB(2), AFG(1) and AFG(2) were detected in 82.5%, 69.37%, 43.12% and 41.87% of the feed samples, respectively. The concentration of AFB(1) in 65% (26/40) and 10% (4/40) of corn silage and straw samples was higher than the European Union limit, respectively. Estimation of the total corresponding concentration of AFM(1) in milk was evaluated as 20.16 and 35.68ng/l during summer and winter, respectively.