Gas chromatography-mass spectrometry (GC-MS) method for the determination of 16 European priority polycyclic aromatic hydrocarbons in smoked meat products and edible oils.

摘要

A GC-MS method was developed for the analysis of 15 PAH highlighted as carcinogenic by the Scientific Committee on Food (SCF) plus benzo[c]fluorine (recommended to be analysed by the Joint FAO/WHO Expert Committee on Food Additives) in fat-containing foods such as oils and smoked meat products. This method includes accelerated solvent extraction and the highly automated clean-up steps gel permeation chromatography and solid-phase extraction. Using a VF-17ms GC column, a good separation of benzo[b]fluoranthene, benzo[j]fluoranthene and benzo[k]fluoranthene was achieved. Furthermore, 6 methylchrysene isomers and PAH compounds with a mol. wt. of 302 Daltons in fat-containing foods attained a better chromatographic separation in comparison with a 5-ms column. The reliability of the analytical technique for oils was demonstrated by the results from a proficiency test. Measurements with GC-high-resolution MS and GC-mass selective detection (GC-MSD) led to comparable results. A survey of 16 PAH in 22 smoked meat products showed concn. in the range <0.01-19 mug/kg. The median concn. for benzo[a]pyrene was below 0.15 mug/kg.