Ultrasensitive detection of enrofloxacin in chicken muscles by surface-enhanced Raman spectroscopy using amino-modified glycidyl methacrylate-ethylene dimethacrylate (GMA-EDMA) powdered porous material.

摘要

Illegally use of enrofloxacin in chicken has raised serious concerns due to its negative effects on public health. In this study, surface-enhanced Raman spectroscopy (SERS) using amino-modified glycidyl methacrylate-ethylene dimethacrylate (GMA-EDMA) powdered porous material was developed and validated to detect enrofloxacin in chicken muscles. By this method, enrofloxacin in chicken muscles was successfully detected at a concentration of 0.01 mg kg-1, which was lower than the legal maximum residue limit. And the unique "fingerprint-like" spectral patterns of enrofloxacin obtained from SERS spectra could be used for identification and characterization. Compared with high-performance liquid chromatography, the assay of six samples with SERS was rapid in less than 40 min, and the detection was highly sensitive. The results demonstrate that SERS using GMA-EDMA powdered porous material can be potentially employed as a screening tool for rapid detection of residual drugs in a large amount of food samples. copyright Springer Science+Business Media New York 2013.