Occurrence of aflatoxin M 1 in some samples of UHT, raw & pasteurized milk from Indian states of Karnataka and Tamilnadu

摘要

Aflatoxin M 1 (AFM 1) is a toxic metabolite found in the milk of lactating animals which have consumed feedstuffs contaminated with aflatoxin B 1. Ultra high temperature treated (UHT) milk is a product which is becoming popular in developing countries like India as there is a lack of proper cold storage or refrigeration facilities. In this study, 45 samples of UHT milk of popular brands prevalent in the market were analyzed for the presence of AFM 1 by reversed phase HPLC using fluorescent detector after cleanup of sample with immunoaffinity columns. All samples of plain UHT milk were positive for AFM 1 and 38% of these contained levels more than 0.5μg/kg, the maximum permitted limit prescribed by the Codex Alimentarius Commission and by the mandatory regulations of the country, the FSSAI Regulations, 2011. In 62.5% of flavored UHT milk, AFM 1 was below detectable levels (0.02μgL -1). However, 12.5% of these samples also contained levels exceeding the maximum permitted limits. AFM 1 was present in 61.6% of the 52 raw milk samples analyzed from the two states of Karnataka and Tamilnadu with a range of 0.1-3.8μgL -1. 17.3% of these samples also exceeded the regulatory limits of the country.