Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms

摘要

A simple, sensitive, and rapid method was developed for the routine identification and quantification of menbutone in different matrices by micellar liquid chromatography. Separation was performed in less than 4 min using a C-18 column with UV detection at 234 nm. A micellar solution composed of 0.12 M sodium dodecyl sulfate, 8 % n-butanol, and 0.3 % triethylamine in 0.02 M phosphoric acid at pH 6.0 was used as the mobile phase. The method was fully validated in accordance with International Conference on Harmonization (ICH) guidelines. The limits of detection and quantitation were 0.95 and 2.86 ng mL(-1), respectively. The method showed good repeatability, linearity, and sensitivity according to the evaluation of the validation parameters. The micellar method was successfully applied for the analysis of menbutone in its commercial injections with a mean % recovery value of 99.73 +/- 1.634 % and in spiked bovine milk and meat samples with a mean % recovery values in the range of 98.00-100.60 %. High extraction efficiency was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, this method can be considered as an interesting example for green chemistry.