Provision of Ultrasensitive Quantitative Gold Immunochromatography for Rapid Monitoring of Olaquindox in Animal Feed and Water Samples

摘要

Olaquindox (OLA), used as a medicinal feed additive, has been put under ban due to hazard concerns over animal-derived food security. In this study, a simple, rapid, ultrasensitive, and quantitative gold immunochromatography assay (GICA) was established to analyze OLA in animal feed samples and surface water samples to monitor food security. Various trying has been experimented to improve the sensitivity. The IC50 of the optimized method is 3.35 mu g L-1 for feedstuff and 0.35 mu g L-1 for environmental water. The recoveries ranged from 77.33 to 86.91 % (CV 23.62 %) for spiked feedstuff and 96.30 to 117.83 % (CV 22.51 %) for spiked water samples. Then, the developed GICA was applied to animal feed and field water, followed by confirmation with ELISA and the consistency of results indicated that the developed GICA could be applied for rapid screening of OLA in real samples. Compared with previous assay, the developed GICA in this study was more sensitive and more rapid, which exhibited broader prospect to supervise the animal products and water rapidly.