DESIGN AND IN-VITRO TESTING OF EFFECTIVE POLY(L-LYSINE /SO-PHTHALAMIDE) BASED DRUG TARGETING SYSTEMS FOR SOLID TUMOURS

摘要

The mode of cell uptake, relative cytotoxicky and efficacy of poly (L-lysine isophthalamide)-drug conjugates, PEG-graft poly (L-lysine wo-phthalamide)-drag containing micelles, poly (L-lysine wo-phthalamide) modified drag/protein nanospheres and unmodified protein nanospheres as potential delivery systems have been evaluated using doxorubicin and polymethine fluorophores as model drugs. pH dependent cell membrane lysis has been demonstrated using a haemolysis model and the cytotoxicity of the polymeric system evaluated using standard MTT and LDH activity assays. In-vitro toxicity and cellular targeting data are presented and the potential healthcare impact of such systems is discussed.