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Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione.

机译:暴露于谷胱甘肽后,冻融的胚胎干细胞的活力提高。

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摘要

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation.
机译:将有效的抗氧化剂谷胱甘肽(GSH)添加到由二甲基亚砜和乙二醇和/或融化后培养基组成的冷冻保护溶液中,可显着改善小鼠胚胎干细胞的融化后生存能力。这种作用是由活性氧的减少引起的,仅在冷冻保存过程中暴露于胚胎干细胞才引起。

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