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Applications of flow cytometry for the detection and characterization of biological aerosols

机译:流式细胞仪在生物气溶胶检测与表征中的应用

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摘要

Flow cytometry (FCM), unlike conventional forms of spectroscopy, allows one to interrogate individual members of a sample population for size, shape, biological, and/or chemical properties. Over the past several years, we have investigated the use of flow cytometry to detect and identify microorganisms in aerosols. The effort to date has shown that changes in scatter patterns or DNA composition suggest that changes in the ambient air are evident. The use of macromolecular dyes and antibodies tagged by fluorescence can be used to identify specific components in the aerosol. Moreover, it is possible to combine several of these modes into one step. The assays presented here take 5 min or less to perform. Strategies for the use of flow cytometry to characterize biological aerosols will be discussed. Both laboratory and field studies that demonstrate the potential use of FCM as a means to detect and characterize microbiological materials in aerosols are discussed. (C) 1999 John Wiley & Sons, Inc.* [References: 21]
机译:与传统形式的光谱学不同,流式细胞术(FCM)可以询问样本群体中各个成员的大小,形状,生物学和/或化学性质。在过去的几年中,我们研究了使用流式细胞仪检测和鉴定气溶胶中的微生物。迄今为止的努力表明,散射模式或DNA组成的变化表明周围空气的变化是明显的。大分子染料和荧光标记抗体的使用可用于鉴定气溶胶中的特定成分。此外,可以将这些模式中的几种组合为一个步骤。此处介绍的分析只需5分钟或更短的时间即可完成。将讨论使用流式细胞仪表征生物气溶胶的策略。讨论了实验室和现场研究,这些研究表明了FCM作为检测和表征气溶胶中微生物物质的手段的潜在用途。 (C)1999 John Wiley&Sons,Inc. * [参考:21]

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