Identification of a Shiga-toxin type I variant containing 14 I an IS1203-like element, from Shiga-toxin producing Escherichia coli O157 : H7

摘要

We found two Ship toxin producing Escherichia coli O157:H7 strains isolated from humans carrying the stx(1) gene with an IS1203-like element (designated as IS1203v(1)). The IS1203v(1) was inserted into the coding region of the A subunit 7 bp upstream from the TGA termination codon, resulting in a loss of two amino acid residues (Ser-Ser) from its C terminus. Toxicity of the Stx1 was confirmed by Vero cell assay. IS1203v(1) hardly affected the stx(1) gene in either its expression or the toxicity of its product. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.