FLO11 expression and lipid biosynthesis are required for air-liquid biofilm formation in a Saccharomyces cerevisiae flor strain

摘要

Air–liquid biolm formation is largely dependent on Flo11p and seems related to cell lipid content and composition. Here, it is shown that in the presence of cerulenin, a known inhibitor of the fatty acid synthase complex, biolm formation is inhibited together with FLO11 transcription in a or strain of Saccharomyces cerevisiae, while the administration of saturated fatty acids to cerulenin-containing medium restores biolm formation and FLO11 transcription. It is also shown that, in biolm cells, the FLO11 transcription is accompanied by the transcription of ACC1, ACS1 and INO1 key genes in lipid biosynthesis and that biolm formation is affected by the lack of inositol in or medium. These results are compatible with the hypothesis that the air–liquid biolm formation depends on FLO11 transcription levels as well as on fatty acids biosynthesis.