LOCALIZATION OF GERMINATION-SPECIFIC SPORE-LYTIC ENZYMES IN CLOSTRIDIUM PERFRINGENS S40 SPORES DETECTED BY IMMUNOELECTRON MICROSCOPY

摘要

The localization of germination-specific spore-lytic enzymes, an amidase and a muramidase, in Clostridium perfringens S40 spores was examined by immunoelectron microscopy with respective antisera raised against the enzymes and a colloidal gold-immunoglobulin G complex. For both antisera, immunogold particles were visualized on the outside of the cortex of dormant spores, and they were not detected in germinated spores and decoated spores. [References: 16]