Effect of Tween 80 and monensin on ruminal fermentation of the diet containing 70% wheat straw treated by white-rot fungus in artificial rumen

摘要

The objective of this study was to investigate the effect of Tween 80 and monensin on rumen fermentation of the diet containing 70% wheat straw treated by. white-rot fungus Pleurotus tuber-regium (TWS-PT) and 30% barley in artificial rumen (RUSITEC). The RUSITEC consisted of four fermentation vessels (V-1, V-2, V-3, V-4 : V-1 was without additives (control), V2 received daily 10 mg of monensin, V3 received daily 0.5% Tween 80 (vol.wt(-1)) and V4 involved the combination of l0mg of monensin with 0.5% Tween 80 (vol.wt(-1)). After an adaptation period (6 days) the fermentation parameters were determined for six consecutive days. Tween 80 did not affect the rumen fermentation of the diet consisting 70% TWS-PT and 30% barley in RUSITEC. Monensin affected the rumen fermentation of the diet by the decreased degradability of DM, neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, cellulose (p < 0.001), the decrease of methane production (p < 0.001) and the higher proportion of propionate within the volatile fatty acids (p < 0.001) in comparison to control. Tween 80 did not improve the potency of monensin. Only some indices of the increase mol% of propionate (about 3.4%) and the decrease of methane production (about 0.47 mmol.day(-1)) were found by using Tween 80 plus monensin in comparison to use of monensin alone.