LncRNA profile study reveals a three-lncRNA signature associated with the survival of patients with oesophageal squamous cell carcinoma

摘要

Background Oesophageal cancer is one of the most deadly forms of cancer worldwide. Long non-coding RNAs (IncRNAs) are often found to have important regulatory roles. Objective To assess the IncRNA expression profile of oesophageal squamous cell carcinoma (OSCC) and identify prognosis-related IncRNAs. Method LncRNA expression profiles were studied by microarray in paired tumour and normal tissues from 119 patients with OSCC and validated by qRT-PCR. The 119 patients were divided randomly into training (n=60) and test (n=59) groups. A prognostic signature was developed from the training group using a random Forest supervised classification algorithm and a nearest shrunken centroid algorithm, then validated in a test group and further, in an independent cohort (n=60). The independence of the signature in survival prediction was evaluated by multivariable Cox regression analysis.