Approaches for gene targeting and targeted geneexpression in plants

摘要

Transgenic science and technology are fundamental to state-of-the-art plant molecular geneticsand crop improvement.The new generation of technology endeavors to introduce genes 'stably' into 'site-specific' locations and in 'single copy' without the integration of extraneous vector 'backbone' sequences or selectable markers and with a 'predictable and consistent' expression. Several similar strategies and technologies, which can push the development of 'smart' genetically modified plants with desirable attributes, as well as enhance their consumer acceptability, are discussed in this review.