Anticipated Mutation Assay Using Single-molecule Real-time (SMRT~(TM)) Sequencing Technology

摘要

I describe here an anticipated mutation assay which estimates the frequency and spectrum of somatic point mutations in any genes of any samples by using SMRT~(TM) (Single Molecule Real Time) DNA sequencing technology, which will be released in 2010. The basic concept of the mutation assay is very simple: just prepare the target tem-plate and sequence it accurately. In this paper, I propose ideas on how to make the template and how to eliminate artifactual mutations caused by damage to the DNA tem-plate.