首页> 外文期刊>Genes and Development: a Journal Devoted to the Molecular Analysis of Gene Expression in Eukaryotes, Prokaryotes, and Viruses >Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N
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Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of CENP-N

机译:着丝粒染色质的结构转变调节CENP-N的细胞周期依赖性募集

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摘要

Specific recognition of centromere-specific histone variant CENP-A-containing chromatin by CENP-N is an essential process in the assembly of the kinetochore complex at centromeres prior to mammalian cell division. However, the mechanisms of CENP-N recruitment to centromeres/kinetochores remain unknown. Here, we show that a CENP-A-specific RG loop (Arg80/Gly81) plays an essential and dual regulatory role in this process. The RG loop assists the formation of a compact "ladder-like" structure of CENP-A chromatin, concealing the loop and thus impairing its role in recruiting CENP-N. Upon G1/S-phase transition, however, centromeric chromatin switches from the compact to an open state, enabling the now exposed RG loop to recruit CENP-N prior to cell division. Our results provide the first insights into the mechanisms by which the recruitment of CENP-N is regulated by the structural transitions between compaction and relaxation of centromeric chromatin during the cell cycle.
机译:CENP-N对着丝粒特异性组蛋白变体含CENP-A的染色质的特异性识别是在哺乳动物细胞分裂之前着丝粒的着丝粒复合体组装中的重要过程。然而,CENP-N募集到着丝粒/线粒体的机制仍然未知。在这里,我们证明了CENP-A特异的RG环(Arg80 / Gly81)在此过程中起着至关重要的双重调控作用。 RG环有助于CENP-A染色质形成紧凑的“阶梯状”结构,从而掩盖了环并因此削弱了其在募集CENP-N中的作用。然而,在G1 / S相过渡后,着丝粒染色质从紧密状态转换为开放状态,从而使现在暴露的RG环能够在细胞分裂之前募集CENP-N。我们的研究结果首次揭示了CENP-N募集的机制,该机制由细胞周期中着丝粒染色质的紧缩和松弛之间的结构过渡来调节。

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