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Analysis of genetic diversity and relationships among waxy maize inbred lines in Korea using SSR markers

机译:利用SSR标记分析韩国糯玉米自交系的遗传多样性及亲缘关系

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Information regarding the genetic diversity and genetic relationships among elite inbred lines is necessary to improve new cultivars in maize breeding programs. In this study, genetic diversity and genetic relationships were investigated among 84 waxy maize inbred lines using 50 SSR markers. A total of 269 alleles were identified at all the loci with an average of 5.38 and a range between 2 and 13 alleles per locus. The gene diversity values varied from 0.383 to 0.923 with an average of 0.641. The cluster tree generated using the described SSR markers recognized two major groups at 32% genetic similarity. Group I included 33 inbred lines while group II included 51 inbred lines. The clustering patterns of most of the waxy maize inbred lines did not clearly agree with their source, pedigree or geographic location. The average GS among all inbred lines was 35.7 +/- 10.8. Analysis of waxy maize inbred lines collected from Korea and China at 50 SSR loci revealed higher values of average number of alleles (4.9) and gene diversity (0.638) in Korean inbred lines as compared to Chinese inbred lines (3.5 and 0.563, respectively). The information obtained from the present studies would be very useful for maize breeding programs in Korea.
机译:关于优良近交系之间的遗传多样性和遗传关系的信息对于改进玉米育种计划的新品种是必要的。在这项研究中,使用50个SSR标记对84个糯玉米自交系的遗传多样性和遗传关系进行了研究。在所有基因座上总共鉴定出269个等位基因,平均为5.38,每个基因座的范围介于2和13个等位基因之间。基因多样性值从0.383到0.923不等,平均值为0.641。使用所描述的SSR标记生成的簇树以32%的遗传相似性识别了两个主要群体。第一组包括33个自交系,而第二组包括51个自交系。大多数蜡质玉米自交系的聚集模式与它们的来源,谱系或地理位置均不明确。所有自交系中的平均GS为35.7 +/- 10.8。分析从韩国和中国在50个SSR基因座处获得的糯玉米自交系,与中国自交系(分别为3.5和0.563)相比,韩国自交系的平均等位基因数目(4.9)和基因多样性(0.638)更高。从本研究中获得的信息对于韩国的玉米育种计划将非常有用。

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