Introduction of multi-alien chromatins carrying different powdery mildew-resistant genes from rye and Haynaldia villosa into wheat genome

摘要

Powdery mildew-resistant wheat lines, Tra. R9002 with rye chromatin and Tra. V149 with Haynaldia villosa chromatin, were crossed to combine their different powdery mildew-resistant genes in the wheat genetic background. In the F5 generation of Tra. R9002 X Tra.V149, a highly powdery mildew-resistant line W159 was selected according to its chromosome number 2n = 41 approx 42. In the F_6 line W159-9 which was immune to powdery mildew, one pair of rye chromosome and one pair of wheat-H. villosa translocated chromosome were identified simultaneously by multicolor fluorescence genomic in situ hybridization in the wheat genetic background (chromosome number 2n = 42). Through C-banding and a sequential C-banding analysis after genomic in situ hybridization, the rye chromosome were identified as 5R and the wheat-H. villosa translocated chromosome were identified as 6VS-6AL. This indicates that W159-9 carries two powdery mildew-resistant genes of Pm4 on 5R and Pm21 on 6VS. The wheat chromosome absent in W159-9 were identified as the 6AS and 5D. On the other hand, seven out of 17 progenies of another F_6 line W159-3 possessed 5R and 6VS-6AL chromosome. These materials could be used for acquiring stable multiple powdery mildew-resistance in wheat breeding programs.