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Differential O-glycosylation in cortical and medullary thymocytes

机译:皮质和髓样胸腺细胞中的差异性O-糖基化

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Differentiation of T lymphocytes is characterized by variable expression of CD8/CD4 co-receptor molecules and changes in the glycosylation pattern. In this work, O-glycosylation was analyzed in microsomes from murine thymocytes purified with the PNA and Amaranthus leucocarpus (ALL) lectins, specific for the T antigen (Gal beta 1,3GalNAc1,0Ser/Thr) in cortical and medullary thymocytes, respectively. Three peptides were used as acceptors for UDP-N-acetylgalactosamine: polypeptide N-acetylgalactosaminyl-transferase (GalNAc transferase)- the peptide motif TTSAPTTS was the best glycosylated one. Cortical ALL-PNA+ thymocytes showed two-fold higher GalNAc transferase activity than ALL+PNA- thymocytes: however. capillary electrophoresis showed a higher proportion of di-versus mono-glycosylated peptides for ALL+PNA- than for ALL+PNA+. We compared the GalNAc transferase activity of thymocytes from dexamethasone-treated mice versus control mice. GalNAc transferase activity was six-fold higher in thymocytes from control mice than from dexamethasone-treated mice: the rate of di-glycosylated peptides for dexamethosone-resistant ALL+ was twofold higher than for ALL- thymocytes. Our results confirm an upregulated biosynthesis of O-glycosidically linked glycans on T cell surface glycoproteins, and suggest that the modification of GalNAc transferase activity plays a relevant role during the maturation process of thymic cells. (c) 2006 Elsevier B.V. All rights reserved.
机译:T淋巴细胞的分化以CD8 ​​/ CD4共受体分子的可变表达和糖基化模式的变化为特征。在这项工作中,分析了分别用皮质和髓质胸腺细胞中的T抗原(Gal beta 1,3GalNAc1,0Ser / Thr)特异性的PNA和and菜(ALL)凝集素纯化的鼠胸腺细胞的微粒体中的O-糖基化。三种肽用作UDP-N-乙酰半乳糖胺的受体:多肽N-乙酰半乳糖胺基转移酶(GalNAc转移酶)-肽基序TTSAPTTS是最好的糖基化肽。皮质ALL-PNA +胸腺细胞显示的GalNAc转移酶活性是ALL + PNA-胸腺细胞的两倍。毛细管电泳显示,ALL + PNA-的双向单糖基化肽比例高于ALL + PNA +。我们比较了地塞米松治疗小鼠与对照小鼠胸腺细胞的GalNAc转移酶活性。对照小鼠的胸腺细胞中GalNAc转移酶活性比地塞米松治疗的小鼠高6倍:地塞米松抗性ALL +的二糖基化肽比率比ALL-胸腺细胞高两倍。我们的结果证实了T细胞表面糖蛋白上O-糖苷连接的聚糖的生物合成上调,并表明GalNAc转移酶活性的修饰在胸腺细胞成熟过程中起着相关的作用。 (c)2006 Elsevier B.V.保留所有权利。

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