Where Does the Oxygen Go? k_La Measurement in Bioreactors

摘要

Small plastic tubes are available as single-use bioreactors for cell cultivation as a cost effective alternative to larger scale cultivation systems. However, the characteristics of the tubes with regard to oxygen transfer have hardly been investigated. Using a specially adapted shake flask reader (SFR) and chemical optical sensors with rapid response time, the volumetric oxygen mass transfer coefficient (k_La) for tube systems was determined. The characteristics of these cultivation vessels were investigated more closely, which represents an important step towards defined screening processes by monitoring oxygen in bioreactors.