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Antibodies: A Tool for Molecular Biology

机译:抗体:分子生物学工具

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摘要

Antibodies are immunoglobulins, which build an important component of an organism's immune system. They are produced by B lymphocytes, a specialized type of white blood cells. When foreign macromolecules enter the body, antibodies are produced to identify and to tag invading macromolecules for elimination by other parts of the immune system. Each antibody recognizes very specifically a particular epitope (a 3 dimensional surface structure build by a certain amino-acid sequence) on its antigen (the foreign macromolecule). This makes antibodies interesting tools in molecular biology because by their aid proteins of interest can be identified in a crude mixture of many proteins. Proteins of interest can be visualized and quantified using antibodies.
机译:抗体是免疫球蛋白,可构成生物体免疫系统的重要组成部分。它们由B淋巴细胞(一种特殊类型的白细胞)产生。当外来大分子进入人体时,会产生抗体来识别和标记入侵的大分子,从而被免疫系统的其他部分清除。每种抗体都非常特异性地识别其抗原(外来大分子)上的特定表位(由某个氨基酸序列构建的3维表面结构)。这使得抗体成为分子生物学中令人感兴趣的工具,因为可以通过它们的帮助在许多蛋白质的粗混合物中鉴定出目标蛋白质。感兴趣的蛋白质可以使用抗体可视化和定量。

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