Three-Dimensional Cultivation of Cells: Rotating Bed Bioreactor Design of System Allows for 3-D Growth

摘要

Cell cultivation is one of the most common techniques used in modern life science and medical research. The predominant method grows cells as mono-layers in plastic dishes. Much of the knowledge about contemporary cell biology has been generated fromsuch 2-D cell culture models. These models are used to shed light on the basic principles of life at the cellular level and on the nature and origin of many diseases. They can also be applied to the study of the influence of drugs on various organisms and to the investigation of the consequences of gene manipulation. Ex vivo culturing mainly takes place in a 2-D world (including research on human stem cells and primary cells for therapeutic applications and tissue engineering).