Antioxidant activity of monooleyl and dioleyl p-coumarates in in vitro and biological model systems

摘要

Lipase-catalysed acidolysis of ￡-coumaric acid with triolein was carried out, followed by separation of the synthesized phenolipids using column chromatography. The identification of synthesized phenolipids was conducted by using HPLC-MS. The antioxidant activities of the purified phenolipids were assessed in in vitro assays and biological systems. Monooleyl and dioleyl j5-coumarates were identified as the phenolipid species present. The prepared phenolipids exhibited varying antioxidant activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and peroxyl radical scavenging, reducing power, p-carotene/linoleate bleaching, human low-density lipoprotein (LDL) cholesterol oxidation as well as hydroxyl and peroxyl radical-induced DNA cleavage assays.