Simultaneous Diagnostic Assay of Catechol and Caffeine Using an in vivo Implanted Neuro Sensor

摘要

Catechol and caffeine were simultaneously analyzed with a bismuth-immobilized carbon nanotube paste electrode (BPE) using square wave (SW) stripping voltammetry. Optimum analytical conditions were determined. Simultaneous working ranges of 100-1,500 mgL~(-1) for caffeine and 5-75 mgL~(-1) for catechol were obtained. In the separated cell systems, a working range of 0.1 -2.1 mgL~(-1) catechol with a correlation coefficient of 0.9935, and a working range of 10-210 mgL~(-1) caffeine with a correlation coefficient of 0.9921 were obtained. A detection limit (S/N) of 0.15 mgL~(-1) (7.7 x 10~(-7)M) and a detection limit of 0.02 mgL~(-1) (1.82 x 10~(-7) M), respectively, manifested for catechol and caffeine. It was found that three macro-type electrode systems could be implanted in fish and rat neuro cells. For both ions, the ion currents were observed. The physiological impulse conditions and the neuronal thinking current were also obtained.