Seleno-methionine decreases biohydrogenation of C18 unsaturated fatty acids in ovine ruminal fluid incubated in vitro with -linolenic acid

摘要

The influence of seleno-methionine (Se-Met) added to an ovine ruminal fluid containing -linolenic acid (-LNA) on the profile of fatty acids (FA) was investigated. The fluid was incubated in vitro at 39 degrees C under CO2 either alone (the control ruminal fluid; RF) or with -LNA (1.67mg/mL) or with a combination of -LNA with either a low (1.25 mu g/mL) or high (4.16 mu g/mL) concentration of Se as Se-Met. Tubes with fluids were removed after 0, 6, 12, 18 or 24h of incubation. -LNA in the fluids with/without Se-Met stimulated the accumulation of C14:0 and C16:0 compared with the RF. The fluids containing Se-Met reduced the concentration of C18:0 compared with the RF. -LNA in the fluids with/without Se-Met increased concentrations of cis9C14:1, cis9C18:1 and trans11C18:1 compared with the RF and the fluids with Se-Met. The fluids with -LNA and Se-Met increased the accumulation of trans9trans11cis15C18:3 compared with the fluids with -LNA. The fluids with -LNA and Se-Met decreased the biohydrogenation to C18:0 compared with the RF. Se-Met in the fluids elevated the concentration of trans11C18:1 and lowered the capacity of the biohydrogenation to C18:0 in the fluids incubated for 24h. Added Se-Met affected the FA profile in incubated fluids.