Differential effects of obesity on acetoacetyl-CoA synthetase gene in rat adipose tissues

摘要

In order to investigate the physiological role of the ketone body-utilizing enzyme acet-oacetyl-CoA synthetase(AACS)in obesity-induced metabolic derangements,we examined the mRNA levels of AACS and other lipogenic enzymes in white adipose tissues(WAT)in the different types of obesity,genetic(Zucker fatty)and high-fat diet(HFD)-induced obesity.Plasma ketone body levels and gene expression levels of AACS and acetyl-CoA carboxylase-2(ACC-2)in WAT were lower in Zucker fatty rats than in lean rats.On the contrary,in HFD-induced obese rats,these levels were increased.Moreover,AACS mRNA expression was affected only in subcutaneous WAT by each type of obesity.Our data suggest that AACS mRNA expression is related to obesity-induced alteration of plasma ketone body levels,and that AACS in subcutaneous WAT plays an important role in the regulation of lipogenesis and ketone body utilization in various obese conditions.